Intradermal Injection with DNA Encoding In Vivo Immunomodulation Following
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چکیده
منابع مشابه
Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host gen...
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InterleUkln (IL-12) protein has been shown to elicit diverse immune logical responses and potent antitumor activity. We demonstrate here that intradermal injection of IL-12 eDNA induces systemic biological effects characteristic ofthe cytokine in vivo. Intradermal Injection ofIL-12 eDNA resulted in local expression of IL-12 mRNA, which correlated with a 10-fold increase in natural killer activi...
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Interleukin (IL-12) protein has been shown to elicit diverse immunological responses and potent antitumor activity. We demonstrate here that intradermal injection of IL-12 cDNA induces systemic biological effects characteristic of the cytokine in vivo. Intradermal injection of IL-12 cDNA resulted in local expression of IL-12 mRNA, which correlated with a 10-fold increase in natural killer activ...
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BACKGROUND This study tested a low-volume (20-30 microl/20-30 microg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation of the injected DNA solution, and a simple delivery system. More than one construct can be injected simultaneously and the method may be combined with adjuvants. RESULT...
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Purpose: The aim of this study was to assay the profile of MCT1 & MCD in mouse unfertilized & fertilized oocytes and preimplantation embryos In vivo and In vitro. Materials and Methods: The presence of mRNAs encoding MCT1 & MCD3 were determined On unfertilized and fertilized oocytes, 2-cell, morulae, blastocyst and cultured embryos in plus glucose KSOM, minus glucose KSOM and pulse glucose KSO...
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